[Foreign genes expression in rat vascular smooth muscle].
Zhonghua Yi Xue Za Zhi
; 74(4): 224-7, 255, 1994 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-7922764
ABSTRACT
Three retroviral vectors containing report gene LacZ or the whole length cDNA of human Pro-UK and tPA, pN2-LacZ, pN2-CMV-ProUK and pN2-CMV-tPA were constructed. The rat vascular smooth muscle cells (VSMCs) were transfected with the plasmids by calcium phosphate coprecipitation or pseudovirus infection. The transfected cells were selected by G418. Southern blot analysis showed that the foreign genes were present in the genome of the transfected VSMC, and the expression products of these genes could be detected in the transfected VSMCs. These results suggested that the VSMC could be a targeting cell for gene therapy on cardiovascular diseases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativador de Plasminogênio Tipo Uroquinase
/
Ativador de Plasminogênio Tecidual
/
Técnicas de Transferência de Genes
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
Zh
Ano de publicação:
1994
Tipo de documento:
Article