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Characterization of the CNRE-1 family of repetitive DNA elements in Cryptococcus neoformans.
Spitzer, S G; Spitzer, E D.
Afiliação
  • Spitzer SG; Department of Pathology, SUNY at Stony Brook 11794-8691.
Gene ; 144(1): 103-6, 1994 Jun 24.
Article em En | MEDLINE | ID: mdl-8026743
The pathogenic yeast Cryptococcus neoformans contains 10-20 dispersed repetitive elements that hybridize to clone CNRE-1.0. Screening of a genomic library with probes derived from CNRE-1.0 identified five phages with restriction maps that overlapped CNRE-1.0 and three additional phages that belonged to two distinct groups. Sequencing of internal 3.5-kb SstI fragments from two CNRE-1-like elements revealed 95% homology, as well as a conserved open reading frame. A PCR-RFLP assay was developed that can distinguish different subfamilies of CNRE-1-like elements.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Fúngico / Sequências Repetitivas de Ácido Nucleico / Cryptococcus neoformans Idioma: En Ano de publicação: 1994 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Fúngico / Sequências Repetitivas de Ácido Nucleico / Cryptococcus neoformans Idioma: En Ano de publicação: 1994 Tipo de documento: Article