[Identification of the beta lactamase R-TEM of Pseudomonas aeruginosa]. / Identification de la beta lactamase R-TEM chez Pseudomonas aeruginosa
Biochimie
; 57(2): 139-43, 1975.
Article
em Fr
| MEDLINE
| ID: mdl-806305
ABSTRACT
This paper is dealing with the enzymatic problem raised by two strains of Ps. aeruginosa resistant to classical beta lactam antibiotics including carbenicillin. These two strains hydrolyse all these antibiotics. In both cases, we have shown the simultaneous biosynthesis of two enzymes an inducible and chromosome cephalosporinase frequently found in this germ, and a constitutive beta lactamase, with a penicillinase activity which has been identified with the extrachromosomic beta lactamase R-TEM. These two enzymes have been separated by affinity chromatography, characterized by their kinetic constants given by computerized microacidimetry, and their isoelectric points which are respectively 9.2 for the cephalosporinase and 5.40 for the penicillinase R-TEM. Isoelectric focussing also shows the separation of these two enzymes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Penicilinase
/
Pseudomonas aeruginosa
/
Cefalosporinase
/
Amidoidrolases
Tipo de estudo:
Diagnostic_studies
Idioma:
Fr
Ano de publicação:
1975
Tipo de documento:
Article