Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.
FEBS Lett
; 341(1): 128-30, 1994 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-8137912
ABSTRACT
Quantitative effects of inhibitors of the replicative DNA polymerases (pol) alpha, delta and epsilon from calf thymus are reported under similar assay conditions. Carbonyldiphosphonate was a competitive inhibitor of pols delta and epsilon, with 4- to 6-fold selectivity compared to pol alpha. Aphidicolin inhibited pols alpha and delta with 6- to 10-fold selectivity compared to pol epsilon. The 'butylphenyl' nucleotides, BuPdGTP and BuAdATP, inhibited pol alpha with at least 1000-fold selectivity compared to pols delta and epsilon. The use of these inhibitors under similar assay conditions permits the discrimination of the three enzymes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Inibidores da Síntese de Ácido Nucleico
/
DNA Polimerase II
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article