Qualitative and quantitative detection of HIV-1 RNA by nucleic acid sequence-based amplification.
AIDS
; 7 Suppl 2: S107-10, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8161439
ABSTRACT
AIM:
To develop a method to detect HIV-1 viral RNA by amplifying a specific nucleic acid sequence.METHOD:
The nucleic acid sequence-based amplification (NASBA) method uses the simultaneous activity of avian myeloblastosis virus reverse transcriptase, T7 RNA polymerase and RNase H to amplify a specific nucleic acid target sequence. VALIDATION An in vitro cultured HIV-1 stock solution was used to validate the NASBA method and determine the variation in RNA measurement.CONCLUSION:
Although NASBA is theoretically capable of specific amplification of RNA or DNA, it is most suitable for amplification of RNA, and therefore for detection of HIV-1 viral RNA.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Virologia
/
RNA Viral
/
HIV-1
Tipo de estudo:
Diagnostic_studies
/
Qualitative_research
Limite:
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article