One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes.
Cancer Res
; 54(9): 2486-91, 1994 May 01.
Article
em En
| MEDLINE
| ID: mdl-8162598
ABSTRACT
We have isolated and ordered yeast artificial chromosomes (YACs) and cosmids surrounding the von Hippel-Lindau (VHL) tumor suppressor and plasma membrane Ca(2+)-transporting ATPase isoform 2 (PMCA-2) genes on chromosome 3p25-26. The YAC contig consists of six YACs and covers a region of 1 megabase. A cosmid-phage contig around VHL and PMCA-2 genes (400 kilobases) was established and integrated into the YAC map. Using these clones, we generated an EcoRI map of the 400-kilobase region. PMCA-2 and VHL complementary DNA were positioned entirely within the cosmid-phage contig as well as two polymorphic markers (D3S601 and D3S1317). This physical map of the cloned region will allow a detailed analysis of both the PMCA-2 and VHL genes. Some of the genomic clones may be useful for isolation of the full-length VHL complementary DNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cosmídeos
/
Genes Supressores de Tumor
/
ATPases Transportadoras de Cálcio
/
Cromossomos Artificiais de Levedura
/
Doença de von Hippel-Lindau
Limite:
Humans
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article