Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells.
Biochem Pharmacol
; 47(9): 1601-6, 1994 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-8185674
ABSTRACT
MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer cell line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance was due to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência a Medicamentos
/
RNA Mensageiro
/
Mitoxantrona
Limite:
Humans
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article