Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants.
Mol Gen Genet
; 243(1): 32-8, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-8190069
ABSTRACT
We have investigated the use of the tetracycline-dependent gene expression system to regenerate and propagate tobacco plants transformed with a gene whose product--when highly expressed--interferes with regeneration and/or further reproduction. Plants transformed with the Agrobacterium rhizogenes rolB gene under the control of the tetracycline-dependent expression system were phenotypically indistinguishable from wild type owing to efficient repression of the promoter. Induction of the rolB gene with tetracycline led to high-level expression of the rolB mRNA, which resulted in extremely stunted plants with necrotic and wrinkled leaves that did not develop a floral meristem. Upon cessation of tetracycline treatment healthy shoots developed even from severely affected meristems. Data on the dose response of the rolB phenotype as a function of tetracycline concentration demonstrate that the tetracycline-dependent gene expression system can be used to modulate the manifestation of a particular phenotype.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Tetraciclina
/
Nicotiana
/
Regulação da Expressão Gênica
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article