[Beef kidney carbonic anhydrases in affinity chromatography and radial immunodiffusion]. / Comportement des anhydrases carboniques du rein de baeuf en chromatographie d'affinité et en immunodiffusion radiale

ABSTRACT

The absence of low activity carbonic anhydrase in the parenchyma of bovine kidney is demonstrated by high specific methods such as affinity chromatography on "CH-Sepharose 4 B"-sulfanilamide and radial immunodiffusion; thus, the high activity carbonic anhydrases C I and C II seem to be the only forms in this tissue. Their identity with the carbonic anhydrases of the erythrocyte suggests that their biosynthesis must be regulated by the same structural gene working alone on these two tissues.