Characterization of an immunodominant Giardia lamblia protein antigen related to alpha giardin.
Parasitol Res
; 79(7): 587-92, 1993.
Article
em En
| MEDLINE
| ID: mdl-8278341
ABSTRACT
The trophozoites of Giardia lamblia possess several protein antigens, predominant among them a protein of approximately 32,000 Da. In the present study, we used monospecific antibodies that recognize this protein to demonstrate its presence on a variety of G. lamblia isolates from human and animal sources. Immune electron microscopy was used to localize 32-kDa antigen on the trophozoite membrane and disk. Immunofluorescent assays employing monospecific antibodies confirmed the presence of 32-kDa antigen on the membrane and disk and its absence on flagella or nuclei. The N-terminal 17 amino acids of the 32-kDa antigen are identical to alpha-1-giardin, a protein component of microribbons on the ventral disk. These results suggest that the 32-kDa immunodominant trophozoite antigen is alpha-1-giardin.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Protozoários
/
Epitopos Imunodominantes
/
Giardia lamblia
/
Proteínas do Citoesqueleto
/
Antígenos de Protozoários
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article