Cellular and metabolic engineering. An overview.
Appl Biochem Biotechnol
; 38(1-2): 105-40, 1993.
Article
em En
| MEDLINE
| ID: mdl-8346901
ABSTRACT
Metabolic engineering is defined as the purposeful modification of intermediary metabolism using recombinant DNA techniques. Cellular engineering, a more inclusive term, is defined as the purposeful modification of cell properties using the same techniques. Examples of cellular and metabolic engineering are divided into five categories 1. Improved production of chemicals already produced by the host organism; 2. Extended substrate range for growth and product formation; 3. Addition of new catabolic activities for degradation of toxic chemicals; 4. Production of chemicals new to the host organism; and 5. Modification of cell properties. Over 100 examples of cellular and metabolic engineering are summarized. Several molecular biological, analytical chemistry, and mathematical and computational tools of relevance to cellular and metabolic engineering are reviewed. The importance of host selection and gene selection is emphasized. Finally, some future directions and emerging areas are presented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Biomédica
/
Células
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article