Construction of a retrotransposition indicator sequence using a neomycin resistance-encoding gene containing a functional intron.
Gene
; 127(2): 233-6, 1993 May 30.
Article
em En
| MEDLINE
| ID: mdl-8388842
ABSTRACT
An intron from a Syrian hamster gene was inserted into a neo gene such that splicing of the neo gene mRNA results in the synthesis of active aminoglycoside phosphotransferase. The unspliced construct is inactive in Escherichia coli, but confers resistance to G418 when transfected into mouse and hamster cells. This selectable marker is designed to aid in the cloning and identification of genomic integration sites following retrotransposition.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfotransferases
/
Retroviridae
/
Elementos de DNA Transponíveis
/
Íntrons
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article