Isolation of a delta 6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp. strain PCC 7120.
Plant Mol Biol
; 22(2): 293-300, 1993 May.
Article
em En
| MEDLINE
| ID: mdl-8389613
ABSTRACT
The enzyme delta 6-desaturase is responsible for the conversion of linoleic acid (182) to gamma-linolenic acid (183 gamma). A cyanobacterial gene encoding delta 6-desaturase was cloned by expression of a Synechocystis genomic cosmid library in Anabaena, a cyanobacterium lacking delta 6-desaturase. Expression of the Synechocystis delta 6-desaturase gene in Anabaena resulted in the accumulation of gamma-linolenic acid (GLA) and octadecatetraenoic acid (184). The predicted 359 amino acid sequence of the Synechocystis delta 6-desaturase shares limited, but significant, sequence similarity with two other reported desaturases. Analysis of three overlapping cosmids revealed a delta 12-desaturase gene linked to the delta 6-desaturase gene. Expression of Synechocystis delta 6- and delta 12-desaturases in Synechococcus, a cyanobacterium deficient in both desaturases, resulted in the production of linoleic acid and gamma-linolenic acid.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Linoleicos
/
Cianobactérias
/
Ácidos Graxos Dessaturases
/
Genes Bacterianos
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article