Determination of the secondary structure of epoxide hydrolase by Raman spectroscopy.
Biochim Biophys Acta
; 1162(1-2): 217-20, 1993 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-8448187
ABSTRACT
The secondary structure of microsomal epoxide hydrolase was determined by Raman spectroscopy and the effect of the membrane microenvironment studied. The ratios of the four secondary structure contents, alpha-helix beta-strandturnundefined, were found to be 47241711 and 58171510 for the solubilized and the membrane-bound epoxide hydrolase, respectively. Based on the spectral analysis in the 2800-2900 cm-1 range, it was concluded that the protein studied produces the disordering effect on the lipid dimyristoylphosphatidylcholine bilayer at 16 degrees C.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epóxido Hidrolases
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article