A simple method for differentiating Leu and Ile in peptides. The negative-ion mass spectra of [M-H]- ions of phenylthiohydantoin Leu and Ile.
Rapid Commun Mass Spectrom
; 9(13): 1241-3, 1995.
Article
em En
| MEDLINE
| ID: mdl-8527818
ABSTRACT
Phenylthiohydantoin (PTH) amino acid derivatives are formed during sequential Edman degradation of peptides and proteins. Isomeric Leu and Ile (which differ only in respectively having iso-butyl and sec-butyl chains, and are often difficult to distinguish by conventional mass spectrometric techniques) may be readily identified by the characteristic decompositions of the [M-H]- ions of their PTH derivatives. The Leu spectrum shows major loss of propane, while that of the Ile derivative shows elimination of both methane and ethane. This method may be used routinely with 10 microgram quantities of peptide material.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Feniltioidantoína
/
Isoleucina
/
Leucina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article