Ultrastructural and biochemical analysis of sperm flagella from an infertile man with a rod-dominant retinal degeneration.
Hum Pathol
; 27(1): 80-4, 1996 Jan.
Article
em En
| MEDLINE
| ID: mdl-8543316
ABSTRACT
This study examined the ultrastructural morphology and posttranslationally modified alpha-tubulin isoforms in the sperm flagella of a patient presenting with infertility and retinal degeneration. Clinical evaluation showed impaired motility and gross morphological abnormalities of the sperm and a rod-dominant retinal degeneration with midperipheral pigment clumping and scattered bone spicules. Other neurological indications included delayed neuroelectric transmission in the auditory brainstem and a temporal lobe seizure disorder. Ultrastructural analysis showed that 46% of sperm axonemes had missing and/or misplaced doublets compared with 10% to 12% in control subjects. ELISA analysis showed hypoacetylation of alpha-tubulin (30% of control) but normal levels of alpha-tubulin tyrosination. Tubulin acetyl-transferase specific activity was also 30% of control activity. These characteristics may be indicative of microtubule instability leading to the pathological consequences described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Degeneração Retiniana
/
Espermatozoides
/
Infertilidade Masculina
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article