CD4-imitating human antibodies in HIV infection and anti-idiotypic vaccination.
J Immunol
; 156(2): 826-33, 1996 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-8543839
ABSTRACT
Anti-idiotypic vaccination against HIV infection aims at inducing an anti-gp120 immune response through anti-CD4 Abs mimicking epitopes of the gp120 molecule. The mAb IOT4a induces anti-gp120 Abs in rabbits. This study investigates the presence of human serum Abs cross-reacting with anti-CD4 mAbs and gp120 during HIV infection and after parenteral vaccination with IOT4a. Ten HIV-infected volunteers without immunodeficiency were inoculated s.c. with 0.6, 1.2, or 2.4 mg IOT4a. Six booster injections followed until day 35. Sera from study patients, 80 HIV-positive, and 43 seronegative controls were examined by a panel of assays, including an ELISA using competition against biotinylated recombinant CD4, flow cytometry assaying inhibition of anti-CD4 mAbs and biotinylated gp120 binding to CD4-positive lymphocytes, and an IOT4a immunoblot. After vaccination, an increase in competitive binding activity, which was quantitative in ELISA and flow cytometry, was observed. In the ELISA, competition against biotin-CD4 was quantitatively quenched by preincubating sera with r-gp120 or anti-CD4 mAbs such as IOT4a and Leu3a. Naive sera or sera from blood donors had no such effect in the assays employed, while 5/80 HIV sera showed binding qualities similar to vaccinees. These results suggest that 1) CD4 internal-image Abs emerge in a small proportion of HIV-positive individuals, and 2) parenteral vaccination with mAb IOT4a can induce a gp120 cross-reacting immune response that inhibits gp120 binding to CD4.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos CD4
/
Proteína gp120 do Envelope de HIV
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Infecções por HIV
/
Anticorpos Anti-Idiotípicos
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Imunoterapia Ativa
/
HIV-1
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Mimetismo Molecular
/
Anticorpos Monoclonais
Limite:
Adult
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Animals
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article