Comparison of four methods for forage nitrate analysis.
J Vet Diagn Invest
; 7(4): 527-30, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-8580177
ABSTRACT
Twenty forage samples were collected and selected for variation in nitrate content. Each forage samples was analyzed 4 times by 4 different methods:
diphenylamine spot plate, spectrophotometric, nitrate-selective electrode, and high-performance liquid chromatographic. Five feed extracts were spiked with 2 different amounts of nitrate and analyzed by each method. The spectrophotometric and nitrate-selective electrode had similar percent recoveries, which were close to 100%. The nitrate-selective electrode method had the least variation of the 4 methods. The diphenylamine spot plate method had the poorest average recovery, greatest variation, and was the least accurate. The average coefficients of variation for all samples within a method were 15%, 12%, 6.4%, and 16 for the diphenylamine spot plate, spectrophotometric, nitrate-selective electrode, and high-performance liquid chromatographic methods, respectively. The variation in the nitrate-selective electrode method was lower (P < 0.05) than the other methods. The results from this study suggest that the nitrate-selective electrode method is more accurate and precise than the other methods of analysis tested.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poaceae
/
Ração Animal
/
Nitratos
Limite:
Animals
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article