Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods.
Toxicol Lett
; 82-83: 883-9, 1995 Dec.
Article
em En
| MEDLINE
| ID: mdl-8597157
ABSTRACT
We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinolinas
/
Imidazóis
/
Mutagênicos
Limite:
Animals
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article