Regulation of the TRP Ca2+ channel by INAD in Drosophila photoreceptors.
Neuron
; 16(5): 991-8, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-8630257
ABSTRACT
Drosophila vision involves a G protein-coupled phospholipase C-mediated signaling pathway that leads to membrane depolarization through activation of Na+ and Ca2+ channels. InaD mutant flies have a M442K point mutation and display a slow recovery of the Ca2+ dependent current. We report that anti-INAD antibodies coimmunoprecipitate TRP, identified by its electrophoretic mobility, cross reactivity with anti-TRP antibody, and absence in a null allele trp mutant. This interaction is abolished by the InaD point mutation in vitro and in vivo. Interaction was localized to the 19 amino acid C-terminus of TRP by overlay assays, and to the PDZ domain of INAD, encompassing the point mutation. Given the impaired electrophysiology of the InaD mutant, this novel interaction suggests that INAD functions as a regulatory subunit of the TRP Ca2+ channel.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Cálcio
/
Células Fotorreceptoras de Invertebrados
/
Proteínas de Insetos
/
Proteínas de Drosophila
/
Proteínas do Olho
/
Hormônios de Inseto
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article