Detection of the gene for toxic shock syndrome toxin 1 in Staphylococcus aureus by enzyme-labelled oligonucleotide probes.
J Med Microbiol
; 44(3): 215-8, 1996 Mar.
Article
em En
| MEDLINE
| ID: mdl-8636940
ABSTRACT
A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test correlated well with those obtained by the reverse passive latex agglutination test. Thus, this method is convenient and reliable for the detection of tst in staphylococci, which could be useful for both research and clinical purposes. This method demonstrated that tst was more prevalent in methicillin-resistant Staphylococcus aureus (56%) than in methicillin-susceptible S. aureus (4%).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Toxinas Bacterianas
/
Técnicas de Sonda Molecular
/
Superantígenos
/
Enterotoxinas
/
Genes Bacterianos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article