Bioprocessing with genetically modified and other organisms: case studies in processing constraints.
Ann N Y Acad Sci
; 782: 391-401, 1996 May 15.
Article
em En
| MEDLINE
| ID: mdl-8659911
ABSTRACT
Whereas the gene stability related considerations are important in bioprocessing with recombinant cultures, bioreactor design and scale-up require attention to the often reduced shear tolerance of the genetically altered biocatalysts relative to the corresponding wild strains. In addition, the peculiarities of expression of the rDNA product impact the downstream recovery methods. As a consequence, a bioprocessing scheme and the process machinery designed for a naturally occurring organism may need significant modifications for use with a genetically modified variety of the same organism. The case studies described highlight some of the processing constraints and consideration of general relevance.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Biotecnologia
/
Proteínas Recombinantes
/
Beta-Galactosidase
/
Técnicas de Cultura
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Eritrócitos
/
Neurospora
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article