Complementation cloning of mammalian transcriptional regulators: the example of MHC class II gene regulators.

ABSTRACT

Cloning by complementation of mutant cell lines is a powerful way in which to identify and isolate important regulatory genes on the basis of functional assays. The recent cloning of two essential regulators of major histocompatibility complex (MHC) class II gene expression has not only advanced our understanding of the complex mechanisms controlling these genes, but also helps to illustrate the feasibility of this approach for the study of mammalian gene regulation.