A rapid and sensitive enzyme linked immunofilter assay (ELIFA) for whole bacterial cells.
J Immunol Methods
; 192(1-2): 133-6, 1996 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-8699009
ABSTRACT
An improved method is described for the detection of Escherichia coli by an enzyme linked immunofilter assay (ELIFA) using nitrocellulose membrane sandwiched between two 96-well plates. The incorporation of a pumping system permits a continuous flow of reagents and/or wash fluids through the membrane and provides an assay procedure capable of detecting 10(3) bacteria per well within 40 min. Quantitative bacterial detection was based on precipitated chromogen determined by scanning densitometry. The procedure represents a significant improvement in assay time and/or sensitivity over previously described ELIFA and ELISA methods for whole bacterial cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Imunoenzimáticas
/
Escherichia coli
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article