Construction of shuttle expression plasmid and stable expression of foreign gene in mycobacteria and E. coli.
J Tongji Med Univ
; 15(3): 138-42, 1995.
Article
em En
| MEDLINE
| ID: mdl-8731940
ABSTRACT
By employing the pUC19 as a backbone, the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Transformação Bacteriana
/
Escherichia coli
/
Vetores Genéticos
/
Mycobacterium
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article