K-ras and p53 mutations in human colorectal aberrant crypt foci.
J Pathol
; 178(3): 259-63, 1996 Mar.
Article
em En
| MEDLINE
| ID: mdl-8778329
ABSTRACT
Aberrant crypt foci (ACF) are putative precursor lesions of colon cancer, recently identified on the methylene blue-stained mucosal surface of human colon. No mutations in K-ras or p53 genes were found by non-radioactive single-strand conformation polymorphism analysis in 14 ACF collected from five patients. Using the more sensitive method of allele-specific polymerase chain reaction (PCR) for K-ras, 8 of 14 ACF were found to contain K-ras mutations, suggesting that mutated cells are present in minute clones in ACF. No dysplasia was observed in any of the ACF containing a mutated clone. The presence of K-ras mutations in ACF suggests that these lesions occur at a very early stage in human colorectal carcinogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesões Pré-Cancerosas
/
Neoplasias Colorretais
/
Genes p53
/
Genes ras
/
Mucosa Intestinal
Limite:
Humans
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article