Endogenous ADP-ribosylation of proteins in Mycobacterium smegmatis.
J Bacteriol
; 178(20): 6074-7, 1996 Oct.
Article
em En
| MEDLINE
| ID: mdl-8830711
ABSTRACT
Endogenous ADP-ribosylation of two proteins with molecular weights of 30,000 (30K) and 80,000 (80K) was detected in cell extracts of Mycobacterium smegmatis. Modification of these proteins was enzymatic. The ADP-ribose bound to 30K was removed by HgCl2 but not by NH2OH, suggesting the modification of a cysteine residue. The ADP-ribose bound to 80K was not removed by either HgCl2 or NH2OH, which is consistent with the modification of an asparagine residue. ADP-ribosylation of 80K appeared to be reversible.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Adenosina Difosfato Ribose
/
Processamento de Proteína Pós-Traducional
/
Poli(ADP-Ribose) Polimerases
/
Mycobacterium
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article