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Residual leukemia and immunomagnetic bead purging in patients with BCR-ABL-positive acute lymphoblastic leukemia.
Atta, J; Martin, H; Bruecher, J; Elsner, S; Wassmann, B; Rode, C; Russ, A; Kvalheim, G; Hoelzer, D.
Afiliação
  • Atta J; Department of Hematology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
Bone Marrow Transplant ; 18(3): 541-8, 1996 Sep.
Article em En | MEDLINE | ID: mdl-8879615
Residual leukemia was evaluated in autologous bone marrow grafts harvested in first (n = 11) or second (n = 3) complete remission from 14 patients with BCR-ABL-positive acute lymphoblastic leukemia after treatment according to the German multicenter ALL protocols. The intervals from diagnosis to BM harvest were median 159 (range 78-463) and from preceding chemotherapy to BM harvest median 39 (range 26-69) days, respectively. A limiting log(10)-dilution RT-PCR was used to semiquantify BCR-ABL-positive cells. All autografts appeared to be significantly contaminated with residual leukemic cells. The BCR-ABL-specific titers ranged from 1:10(3) to 1:10(6) (median 1:10(4)) above the limit of detection. This was the rationale to purge the grafts using two cycles of IgM anti-CD10, CD19, and AB4 MoAbs-coated immunomagnetic beads (IMB). Purging depleted median 3 (range 2-4) logs of residual leukemia, resulting in a median 1:10(1) (range 1:10(0) to 1:10(3)) postpurge BCR-ABL-specific titer. The second purging cycle accounted for 1 log of depletion. The mean +/- s.e.m. post-purge recoveries of MNC and CFU-GM were 59 +/- 4%, and 61 +/- 9%, respectively. We conclude that all BCR-ABL-positive ALL patients achieving CR by cytological criteria have critically high levels of residual leukemia in their bone marrow, which can be reduced by median 3 log using immunomagnetic bead purging.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Fusão bcr-abl / Transplante de Medula Óssea / Purging da Medula Óssea / Separação Imunomagnética / Leucemia-Linfoma Linfoblástico de Células Precursoras Tipo de estudo: Guideline Limite: Adolescent / Adult / Child / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 1996 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Fusão bcr-abl / Transplante de Medula Óssea / Purging da Medula Óssea / Separação Imunomagnética / Leucemia-Linfoma Linfoblástico de Células Precursoras Tipo de estudo: Guideline Limite: Adolescent / Adult / Child / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 1996 Tipo de documento: Article