Radioreceptor assay for sirolimus.
Clin Biochem
; 29(5): 457-60, 1996 Oct.
Article
em En
| MEDLINE
| ID: mdl-8884067
ABSTRACT
OBJECTIVES:
To develop a radioreceptor assay (RRA) for sirolimus (rapamycin, RAPA).METHODS:
A direct methanol extraction was used to prepare 45 patient samples for the RRA. Results were compared to the results obtained previously using high-performance liquid chromatography (HPLC). Between-run precision, recovery, and drug interference studies were also performed.RESULTS:
The RRA is sensitive to 1.0 microgram/L RAPA equivalents in whole blood. Comparison with HPLC yielded a correlation coefficient for 45 patient samples of 0.977. Between-run precision at 2.5, 7.5, 12.5, and 20 micrograms/L showed coefficients of variation (CVs) of 12.9, 9.2, 8.5, and 5.9%, respectively. Recoveries from the extraction procedure were 93% at 7.5 micrograms/L and 103% at 12.5 micrograms/L. Drug interference studies showed no interference in the RRA by cyclosporine (CsA), dexamethasone, prednisone, or methotrexate.CONCLUSION:
We have demonstrated that the RRA for RAPA correlates well with HPLC, and has excellent precision and recovery. The procedure is far less time-consuming and complex than HPLC and has potential for automation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polienos
/
Ensaio Radioligante
/
Imunossupressores
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article