Comparison of allergic aspermatogenesis with that induced by vasectomy. II. In vitro studies of cell-mediated immunity to sperm after vasectomy in man and guinea-pig.
Clin Exp Immunol
; 28(3): 461-6, 1977 Jun.
Article
em En
| MEDLINE
| ID: mdl-891022
ABSTRACT
Transformation of peripheral blood leucocytes was shown to be a valid assay for cell-mediated immunity to sperm in male guinea-pigs immunized with homologous epididymal sperm (ES) in FCA. A heat-treated extract of ES (BES) was used for culture. Possible developement of cell-mediated immunity to sperm after vasectomy was investigated in patients and guinea-pigs, by culture of blood leucocytes before and at intervals after operation. Patients' leucocytes were cultured with a heat-treated extract of human seminal sperm (BHS); guinea-pigs' leucocytes were cultured with BES. These cultures showed no evidence of specific stimulation of leucocytes by sperm extract, up to 1 year after vasectomy in patients, or up to 6 months in guinea-pigs. Similarly, no evidence of delayed hypersensitivity could be demonstrated by skin tests with BES in animals vasectomized 1 year previously. We consider that this study establishes that cell-mediated immunity to sperm does not develop in either man or guinea-pigs, up to 1 year after conventional vasectomy.
ABSTRACT
PIP In vitro studies of cell-mediated immunity to sperm after vasectomy in man and guinea-pig are reported. Transformation of peripheral blood leucocytes was shown to be a valid assay for cell-mediated immunity to sperm in male guinea-pigs immunized with homologous epididymal sperm (heat treated extract-BES) in FCA. Patients' leucocytes cultured with a heat-treated extract of human seminal sperm, and guinea-pigs' leucocytes cultured with BES revealed no evidence of specific stimulation by sperm extract up to 1 year and 6 months postvasectomy, respectively. Also, no evidence of delayed hypersensitivity could be seen by skin tests with BES in animals vasectomized 1 year previously. It is concluded that cell-mediated immunity to sperm does not develop in man or guinea pigs up to 1 year postvasectomy.
Palavras-chave
Animals, Laboratory; Biology; Clinical Research; Comparative Studies; Examinations And Diagnoses; Family Planning; Genitalia; Germ Cells; Immunity; Immunological Effects; In Vitro; Laboratory Examinations And Diagnoses; Laboratory Procedures; Male Sterilization; Physiology; Research Methodology; Spermatozoa; Sterilization, Sexual; Studies; Urogenital System; Vasectomy--side effects
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Vasectomia
/
Imunidade Celular
Limite:
Animals
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Humans
/
Male
Idioma:
En
Ano de publicação:
1977
Tipo de documento:
Article