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Annexin II modulates volume-activated chloride currents in vascular endothelial cells.
Nilius, B; Gerke, V; Prenen, J; Szücs, G; Heinke, S; Weber, K; Droogmans, G.
Afiliação
  • Nilius B; Laboratorium voor Fysiologie, KU Leuven, B-3000 Leuven, Belgium. bernd.nilius@med.kuleuven.ac.be
J Biol Chem ; 271(48): 30631-6, 1996 Nov 29.
Article em En | MEDLINE | ID: mdl-8940038
The membrane-associated, microfilament-binding protein annexin II is abundantly expressed in endothelial cells from calf pulmonary artery (CPAE cells). We have analyzed its role in the regulation of volume-activated chloride currents (ICl, vol) by loading the cells via the patch pipette with a peptide comprising the N-terminal 14 residues of annexin II. This sequence harbors the binding site for the intracellular annexin II ligand, p11, and the peptide interferes with the annexin II-p11 complex formation. Loading of a CPAE cell with this peptide caused a gradual decrease in the amplitude of ICl, vol during repetitive stimulations with a 28% hypotonic extracellular solution. This run down of the current was virtually absent in untreated cells and in cells that were loaded with a mutated 14-amino acid peptide, which has a single amino acid replacement known to result in a more than 1000 times reduced affinity for binding to p11. We conclude that annexin II-p11 complex formation is either directly or indirectly involved in the activation of ICl, vol in endothelial cells.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Vascular / Proteínas S100 / Cloretos / Anexina A2 / Canais de Cloreto Limite: Animals Idioma: En Ano de publicação: 1996 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Vascular / Proteínas S100 / Cloretos / Anexina A2 / Canais de Cloreto Limite: Animals Idioma: En Ano de publicação: 1996 Tipo de documento: Article