Complement activation in human serum by liposome-encapsulated hemoglobin: the role of natural anti-phospholipid antibodies.
Biochim Biophys Acta
; 1285(2): 127-30, 1996 Dec 04.
Article
em En
| MEDLINE
| ID: mdl-8972696
ABSTRACT
In exploring the occurrence and mechanism of liposome-encapsulated hemoglobin (LEH)-induced complement (C) activation, we found that normal human serum contained low titers of IgG and IgM class natural antibodies with reactivity against LEH, and that the amount of vesicle-bound IgM significantly correlated with LEH-induced C consumption. IgM binding to LEH was inhibited by phosphocholine and ATP, but not by choline chloride. These data suggest that naturally occurring antibodies play a key role in LEH-induced C activation, and that a major portion of these antibodies are directed against the phosphate moiety on the phospholipid headgroups of liposome bilayers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemoglobinas
/
Ativação do Complemento
/
Lipossomos
/
Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article