Additional data about thermolysin specificity in buffer- and glycerol-containing media.
Biochim Biophys Acta
; 1337(1): 143-8, 1997 Jan 04.
Article
em En
| MEDLINE
| ID: mdl-9003446
ABSTRACT
Synthesis and use of various substrates permit an improved approach to thermolysin-peptide recognition and elucidation of several new criteria affecting enzyme specificity. Nature and position of the recognized residue, role of adjacent amino acids, lateral chain hydrophobicity, and volume and length of peptides were all considered. Hydrolysis reactions were also carried out in the presence of glycerol; the effect of microenvironment modifications was quantitative, for example in inducing variations in catalytic reaction rates, and also qualitative, such as in influencing affinity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Termolisina
Tipo de estudo:
Qualitative_research
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article