Improvement in establishing the period of rubella virus primary infection using a mild protein denaturant.
J Virol Methods
; 66(1): 109-12, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9220396
A more precise determination of the date of primary rubella infection in pregnancy is obtained by application of the urea denaturation method. Two denaturation buffers containing 6 and 8 M urea were compared for the detection of rubella IgG avidity by enzyme immunoassay. The results demonstrate that IgG avidity detected by 6M urea increases more rapidly, from 16% on the third day to 51% on the 46th day after the rash, than that obtained by 8 M urea, from 13 to 36%. The specific rubella IgG avidity detected by 6 M urea denaturation buffer increased significantly in paired sera obtained at an interval of 15 days. The same samples did not show any significant increase of IgG avidity when an 8 M urea denaturation buffer was used. The use of a mild denaturant such as 6 M urea indicates the presumptive date of primary rubella infection in pregnancy within 2 months of sampling.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complicações Infecciosas na Gravidez
/
Rubéola (Sarampo Alemão)
/
Anticorpos Antivirais
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article