Centrifugation does not alter spatial distribution of 'BEP4' mRNA in paracentrotus lividus EGG.
FEBS Lett
; 410(2-3): 499-501, 1997 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-9237691
ABSTRACT
Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
RNA Mensageiro
/
Centrifugação com Gradiente de Concentração
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article