Effect of allopurinol on lipid peroxidation induced in corporeal tissue by veno-occlusive priapism in a rat model.
Br J Urol
; 80(3): 476-9, 1997 Sep.
Article
em En
| MEDLINE
| ID: mdl-9313672
ABSTRACT
OBJECTIVE:
To investigate the role of allopurinol in the attenuation of ischaemia- and reperfusion-induced corporeal injury in a rat model of veno-occlusive priapism. MATERIALS ANDMETHODS:
Placebo or allopurinol were given to eight groups of rats before priapism (ischaemia) was induced using a vacuum-constriction device for a duration of 6 or 12 h. Half of the groups of rats undergoing the same duration of priapism had 1 h of detumescence after the constriction band was removed (reperfusion). A ninth group was not treated and received no drug, serving as controls. Corporeal homogenates were then examined for malondialdehyde (MDA) accumulation, derived from lipid peroxidation, using a thiobarbituric acid assay.RESULTS:
The accumulation of MDA was significantly higher in the groups treated with placebo and undergoing ischaemia/reperfusion compared with the control group (P < 0.001), but was not significantly different between the placebo-treated ischaemic and reperfused groups (P > 0.05). Rats undergoing 6 and 12 h of ischaemia and reperfusion, and receiving allopurinol had significantly less accumulation of MDA compared with those receiving placebo (P < 0.005).CONCLUSIONS:
Lipid peroxidation, an indicator of injury induced by reactive oxygen metabolites, occurred in corporeal tissue during and after veno-occlusive priapism in this rat model; when assessed by lipid peroxidation, allopurinol appeared to protect rat corporeal tissue against this injury.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Priapismo
/
Peroxidação de Lipídeos
/
Traumatismo por Reperfusão
/
Alopurinol
/
Antimetabólitos
Limite:
Animals
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article