Demonstration of concanavalin A receptors on Leptomonas pessoai cell memebrane.
J Protozool
; 23(2): 329-33, 1976 May.
Article
em En
| MEDLINE
| ID: mdl-933086
ABSTRACT
Leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin A (ConA). Agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-D-mannoside. Cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. A Con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect Con A receptors in the pellicular membrane of the flagellar pocket region and in the flagellar membrane proper. Somewhat less of the Con A-DAB reaction product was observed in the pellicular membrane enclosing the rest of the cell.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Concanavalina A
/
Eucariotos
Limite:
Animals
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article