Reconstruction of peritoneal-like structure in three-dimensional collagen gel matrix culture.
Exp Cell Res
; 236(1): 155-60, 1997 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-9344595
ABSTRACT
The peritoneum is a serous membrane consisting of different kinds of cells and extracellular matrix components (ECM). The aim of the present study was to develop a three-dimensional (3D) in vitro culture system for possible investigation of pathological conditions of the peritoneum. Human omental mesothelial cells (MC) and endothelial cells from the umbilical vein (EC) were cultivated either on (MC) or in (EC) a preformed type I collagen matrix. In 3D culture mesothelial cells showed their phenotypical in vivo characteristics and the synthesis of a new basal membrane (BM). Endothelial cells developed vessel-like structures, produce a BM and express E-selectin after TNF-alpha stimulation. This 3D culture system presents extended possibilities for analyzing mesothelial and endothelial cell behavior as well as the cell-cell and cell-matrix interactions involved in several pathological processes in the peritoneum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Omento
/
Peritônio
/
Proteínas da Matriz Extracelular
/
Colágeno
/
Técnicas de Cultura de Células
Limite:
Humans
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article