Simultaneous quantification of cefotaxime, desacetylcefotaxime, ofloxacine and ciprofloxacine in ocular aqueous humor and in plasma by high-performance liquid chromatography.
J Chromatogr B Biomed Sci Appl
; 700(1-2): 147-53, 1997 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-9390724
ABSTRACT
Cefotaxime, given intravenously, is currently used as a broad-spectrum antibiotic for prophylaxis of intra- and postoperative infections in ocular lens surgery. A proposed therapeutic and economic alternative is the use of orally active fluoroquinolone ofloxacine as prophylactic agent. A HPLC method was developed for determination of both antibiotics in ocular aqueous humor and plasma in order to optimize dosage for safe surpassing minimal inhibitory concentration in the humor compartment. For plasma determinations a solid-phase extraction procedure was used with ciprofloxacine as internal standard. Detection limits for direct HPLC-analysis of ocular aqueous humor was 0.08 microg/ml for all compounds, whereas in plasma 0.31 microg/ml could be determined after solid-phase extraction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Humor Aquoso
/
Ofloxacino
/
Ciprofloxacina
/
Cefotaxima
/
Antibacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article