Analysis of protein aggregates by combination of cross-linking reactions and chromatographic separations.
J Chromatogr B Biomed Sci Appl
; 699(1-2): 439-61, 1997 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-9392387
ABSTRACT
Chemical cross-linking provides a method that covalently bridges near-neighbour associations within proteins and protein aggregates. Combined with chromatographic separations and protein-chemical methods, it may be used to localize and to investigate three-dimensional relations as present under natural conditions. This paper reviews the chemistry and application of cross-linking reagents and the development of combination experimental approaches in view of chromatographic separations and cross-linking reactions. Investigations of homooligomeric and heterooligomeric protein associations as well as conformational analysis are presented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Cromatografia Líquida
/
Reagentes de Ligações Cruzadas
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article