Molecular markers for differentiation of multiresistant Klebsiella pneumoniae isolates in a pediatric hospital.
Infect Control Hosp Epidemiol
; 18(11): 743-8, 1997 Nov.
Article
em En
| MEDLINE
| ID: mdl-9397367
ABSTRACT
OBJECTIVE:
To study the spread of extended-spectrum beta-lactamase-producing, but aminoglycoside-susceptible, Klebsiella pneumoniae strains in our hospital over an 8-month period, by using two genotypic markers.DESIGN:
Ribotyping (using two endonucleases) and randomly amplified polymorphic DNA analysis (RAPD; using two different 10-mer primers) were applied to the epidemiological typing of clinical K pneumoniae isolates from stools, ileal fluid, or urine of hospitalized children. SETTING AND PATIENTS The surgical intensive-care ward (S1 9 patients, 17 isolates), surgical unit (S2 2 patients, 2 isolates), and gastroenterology ward (GE 1 patient, 1 isolate) of the Robert Debré Hospital of Paris, France.RESULTS:
Ribotyping of the 20 clinical isolates, the type strain of the species, and two epidemiologically unrelated isolates with EcoRI and HindIII revealed 6 and 5 different patterns, respectively. Six ribotypes were identified by using these two enzymes. RAPD generated 6 distinct patterns, in complete agreement with ribotyping. Our genotypic results showed that 11 patients from wards S1, S2, and GE harbored genotypically related strains, suggesting nosocomial transmission and cross-colonization between and within the three wards.CONCLUSIONS:
Ribotyping and RAPD appear to be reliable methods for distinguishing K pneumoniae strains. The spread of one strain of K pneumoniae in different units of our hospital was demonstrated by both methods. However, RAPD has the advantage of simplicity and rapidity conferred by polymerase chain reaction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Klebsiella
/
Infecção Hospitalar
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Klebsiella pneumoniae
Limite:
Female
/
Humans
/
Infant
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Male
/
Newborn
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article