Studies towards a disposable screen-printed amperometric biosensor for progesterone.
Biosens Bioelectron
; 12(11): 1113-21, 1997.
Article
em En
| MEDLINE
| ID: mdl-9451799
ABSTRACT
A screen-printed carbon electrode (SPCE) has been investigated as the base transducer for a disposable amperometric progesterone biosensor. The biorecognition element was a monoclonal sheep anti-progesterone antibody (mAb). This was immobilized onto the transducer by interaction with a layer of rabbit IgG which had been previously coated onto the SPCE; optimum conditions for these loadings were deduced experimentally. The device was employed in a competitive assay using alkaline phosphatase-labelled progester-one. Three possible substrates for the enzyme were considered, namely, phenyl phosphate, phenolphthalein phosphate and 4-aminophenol phosphate. Cyclic voltammetry and amperometry were carried out on the corresponding aromatic phenols and phenol itself was found to give the best electrochemical characteristics; consequently, phenyl phosphate was employed as the substrate. Chronoamperometry was used to measure the phenol produced by the reaction of bound enzyme-labelled progesterone and substrate. The chronoamperometric response was dependent on unlabelled progesterone over at least three orders of magnitude with a detection limit of about 1 x 10(-9) mol/dm3. This suggests that the device may have applications for the analysis of biological fluids.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Progesterona
/
Técnicas Biossensoriais
/
Equipamentos Descartáveis
/
Eletroquímica
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article