Expression of the human immunodeficiency virus gag gene products by a replication-incompetent herpes simplex virus vector.
Virus Res
; 54(1): 31-8, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9660069
ABSTRACT
A recombinant replication-incompetent herpes simplex virus vector (vd120/Gag) that expressed the human immunodeficiency virus 1 gag gene and part of the pol gene that encodes the HIV-1 protease was constructed. Examination of cells infected with vd120/Gag revealed the presence of the Gag polyprotein Pr55gag by 12 h post-infection, as well as abundant levels of the proteolytically processed 24-kDa capsid protein. Analysis of vector-infected cells and culture supernatant indicated that the majority of the 24-kDa protein remained cell-associated. Although the HIV-1 Gag polyprotein was produced in vd120/Gag-infected cells, there was no evidence of HIV virus-like particle production upon examination of vector-infected cells by transmission electron microscopy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes gag
/
HIV-1
/
Herpesvirus Humano 1
/
Vetores Genéticos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article