Isolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms.
Anim Genet
; 29(2): 91-7, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9699268
ABSTRACT
The present paper describes the isolation and linkage mapping of two isoforms of skeletal muscle myosin heavy chain in pig. Two partial cDNAs (pAZMY4 and pAZMY7), coding for the porcine myosin heavy chain-2B and -beta respectively, have been isolated from a pig skeletal muscle cDNA library. Four RFLPs were detected with the putative porcine skeletal myosin heavy chain-2B probe (pAZMY4) and one RFLP was identified with the putative myosin heavy chain-beta probe (pAZMY7). Two myosin heavy chain loci were mapped by linkage analysis performed with the five RFLPs against the PiGMaP linkage consortium ResPig database the MYH1 locus, which identifies the fast skeletal muscle myosin heavy chain gene cluster, was located at the end of the map of porcine chromosome 12, while the MYH7 locus, which identifies the myosin heavy chain-alpha/-beta gene cluster, was assigned to the long arm of porcine chromosome 7.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Mapeamento Cromossômico
/
DNA Complementar
/
Músculo Esquelético
/
Cadeias Pesadas de Miosina
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article