Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy.
FEBS Lett
; 434(3): 255-60, 1998 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-9742934
ABSTRACT
The Brassica pollen allergen Bra r 1 belongs to a new family of Ca2+-binding proteins, characterized by the presence of two potential EF-hand calcium-binding domains. Disruption of these EF-hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca2+-binding sites. Calcium-binding deficient mutants displayed significantly reduced IgE-binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pólen
/
Brassica
/
Alérgenos
/
Imunoterapia
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article