Horizontal cells of the rat retina show choline acetyltransferase- and vesicular acetylcholine transporter-like immunoreactivities during early postnatal developmental stages.
Neurosci Lett
; 253(2): 83-6, 1998 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-9774155
ABSTRACT
We examined cholinergic neurons in the developing rat retina; an antiserum against choline acetyltransferase (ChAT) and an antiserum against vesicular acetylcholine transporter (VAChT) were used. From postnatal day 4 (P4) to P10, ChAT- and VAChT-like immunoreactivities were seen in cells which were located in the outer part of the inner nuclear layer. These cells had relatively large cell bodies and extended several transversely oriented processes. Double fluorescence immunohistochemistry using an antiserum against calbindin D-28K, a specific marker for the horizontal cells, revealed that all of ChAT- or VAChT-labeled cells showed calbindin D-28K-like immunoreactivity. These cells were no longer immunostained after P11. Thus, acetylcholine was considered to be transiently synthesized in the horizontal cells during early postnatal developmental stages in the rat retina.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Retina
/
Proteínas de Transporte
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Colina O-Acetiltransferase
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Proteínas de Transporte Vesicular
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Animais Recém-Nascidos
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article