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Use of antisense oligodeoxynucleotides to inhibit expression of glutamate transporter subtypes.
Bristol, L A; Rothstein, J D.
Afiliação
  • Bristol LA; Department of Neurology, Johns Hopkins University, Baltimore, Maryland 21287, USA.
Methods Enzymol ; 296: 514-29, 1998.
Article em En | MEDLINE | ID: mdl-9779471
Antisense ODN are highly effective tools to selectively inhibit synthesis of glutamate transporter subtypes. They have been used to evaluate the biology of individual transporter subtypes in vivo and in vitro. Appropriate use of antisense ODN, however, requires a number of important controls to validate the specificity of their effects. Ultimately, the efficacy at inhibiting the synthesis of transporters proteins reflects several variables: the actual efficacy of the antisense to directly inhibit synthesis, the rate of degradation of the ODN, the rate of intracellular penetration of the ODN, the rate of new protein synthesis for the transporter subtype, and the actual penetration of the ODN into CNS tissue. The methods described in this chapter will help one to optimize each of these parameters.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Glutâmico / Transportadores de Cassetes de Ligação de ATP / Corpo Estriado / Oligodesoxirribonucleotídeos Antissenso / Simportadores Limite: Animals Idioma: En Ano de publicação: 1998 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Glutâmico / Transportadores de Cassetes de Ligação de ATP / Corpo Estriado / Oligodesoxirribonucleotídeos Antissenso / Simportadores Limite: Animals Idioma: En Ano de publicação: 1998 Tipo de documento: Article