NPY messenger RNA is increased in medial hypothalamus of anorectic tumor-bearing rats.
Regul Pept
; 75-76: 347-53, 1998 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-9802428
ABSTRACT
Previous investigations suggest that neuropeptide Y (NPY) feeding mechanisms and corticotropin releasing factor (CRF) are altered in anorectic tumor-bearing (TB) rats. To better determine the relationship of NPY and CRF synthesis to cancer anorexia we measured mRNA for these peptides in medial and lateral hypothalamus of TB and control rats. NPY and CRF mRNA were reliably detected by Northern blot analysis only in medial hypothalamus, where NPY message was elevated significantly in TB rats. CRF mRNA tended to be reduced in both pair-fed (PF) and TB rats, but did not reach statistical significance. Concentrations of NPY or CRF were not altered significantly in either the lateral or medial hypothalamus of TB or PF rats. These results suggest that the transcription of NPY is elevated in PF rats and is increased further in anorectic TB rats. The lack of significant increases in levels of peptides may be related to dilution, due to measuring a relatively large block of hypothalamic tissue. Alternatively, translation of the signal for NPY production may be inhibited, or degradation of peptide levels may be increased.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sarcoma Experimental
/
Neuropeptídeo Y
/
RNA Mensageiro
/
Anorexia
/
Hipotálamo Médio
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article