Production of antibody against saikosaponin a, an active component of bupleuri radix.
Arch Pharm Res
; 21(2): 135-9, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9875420
ABSTRACT
High titer rabbit polyclonal antibodies (pAbs) which show a specificity for saikosaponin a (SSA), have been generated. The immunogen used was a conjugate of SSA linked through its glucose moiety to bovine serum albumin by periodate oxidation method. The antibody titers obtained from two rabbits, inoculated with the immunogen, reached a plateau after the fourth and third booster injection, respectively. The specificity of the pAbs was determined by hapten inhibition assays using several SSA-like structures. SSA competitively inhibited the binding of the rabbit anti-SSA pAbs to SSA-ovalbumin on solid phase, a coated antigen on the well. The antibodies showed high specificity to SSA, exhibiting no significant cross-reactivity with any of SSA analogues tested.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Oleanólico
/
Plantas Medicinais
/
Sapogeninas
/
Saponinas
/
Imunossupressores
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article