In vitro angiogenic activity of Aloe vera gel on calf pulmonary artery endothelial (CPAE) cells.
Arch Pharm Res
; 21(3): 260-5, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-9875441
ABSTRACT
Angiogenic activity of Aloe vera gel was investigated by in vitro assay. We obtained the most active fraction from dichloromethane extract of Aloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type 1 collagen gel and form capillary-like tube through in vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel through in vitro invasion assay. Furthermore, the effect on the mRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitor-1 (PAI-1) mRNA was not changed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Artéria Pulmonar
/
Endotélio Vascular
/
Neovascularização Fisiológica
/
Aloe
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article